Genetic variability and biodiversity of Ukrainian Gray cattle by the BoLA-DRB3 gene

At the current stage of genetic studies cattle, more and attention is being drawn to autochthonous breeds. Native cattle have a number prominent phenotypic traits preserved unique genes their combinations lost by modern commercial breeds, which would be valuable use in selective programs. We surveyed polymorphism Ukrainian Gray breed according alleles exon 2 BoLA-DRB3 gene. The uniqueness gene lies broad variability its allele variants. Significant informativeness at DNA level quite important for studies. using PCR-RLFP method on isolated from 88 samples blood cows 5 sperm. identified 28 alleles, 23 variants were nomenclature ones (jba, *jab, *jbb, *nad *nda) “without established nomenclature”, share accounting 8.9%. Four *06, *12, *16 *jba had frequency above 5% occupied 69.9% breed’s fund overall. commonest was BoLA-DRB3.2*16 (44.1%). In total, we found 40 genotypes. Considering significant dominance variant *16, as expected, genotypes with inclusion occurred: *16/*16, *12/*16, *06/*16, *16/*24 *jba/*16. It present genotype two out three studied animals. Parameters heterozygosity, effective Shannon Pielou indices indicate that are characterized lowest biodiversity compared other Due has no inbred motifs. noted deviation toward increase homozygosity without deviations norm distribution Hardy-Weinberg equilibrium. obtained results will used genetic-populational programs purpose improving potential breeds terms economically beneficial diseases cattle.